About second situation, i hence you can expect to differentiate each other main spots and you can ?step 1 plot on every area of the membranous cells ( Contour step 1 and you will Dining table step 1)
From inside the Yards. seminigra queens, i observed each other single couple and you will multiple pairs off melanized spots ( Figure step one and Additional Shape S4 ), and then we never ever found permanent mating plugs within this varieties. New shipments out of multiple patches ranged ranging from step 3 and you can eight custom parts, with different kind of plans. Throughout the 33 M. seminigra specimens analyzed, 15 shown unmarried paired patches trend, twelve demonstrated one coordinated spots in combination with a main patch, and you will 6 specimens presented a multiple patch trend ( Additional Shape S4 ). During these arrangements, this new central melanized spots matched brand new inferred standing of your own spatha-this new main structure on the mating plug ( Contour 2E,I). It’s really worth bringing-up that people seized a supplementary newly mated Meters. seminigra king, which was holding 2 mating plugs: step one try connected inside her vaginal chamber, as the almost every other is onto the additional skin off the girl instinct, ranging from sterna IV and you can V ( Secondary Contour S4A ). So it freshly mated wasn’t totally physogastric, which means that wasn’t utilized in all of our main dataset.
Male vaginal medicine and vaginal sternum regarding M. fasciculata. Ventral look at vaginal pill, metasomal sternum S-VIII, and you can metasomal sternum S-IX, since the discovered into the virgin men (A). Ventral look at metasomal sternum S-VIII (B) and you may metasomal sternum S-IX (C). Vaginal capsule within the ventral look at, exhibiting the newest gonocoxites and you can a couple of gonostyli (D). Genital medicine inside the dorsal examine, to present the new spatha and you will a set of penis valves (E). Genital capsule from inside the ventral consider, indicating into the series, the fresh rotation of penis regulators, ahead of gonocoxites horizontal compressing (F–H). Vaginal product that have caused dick valves and you will gonocoxites compressed laterally, inside the dorsal (I) and you will ventral evaluate (J).
Male genital capsule and you can genital sternum out-of Yards. fasciculata. Ventral look at vaginal capsule, metasomal sternum S-VIII, and metasomal sternum S-IX, since the receive to the virgin men (A). Ventral look at metasomal sternum S-VIII (B) and you will metasomal sternum S-IX (C). Genital pill into the ventral consider, exhibiting the fresh new gonocoxites and you will a pair of gonostyli (D). Vaginal medicine into the dorsal look at, to present the fresh spatha and you may a couple of knob valves (E). Genital pill from inside the ventral have a look at, indicating in the succession, the newest rotation of knob regulators, just before gonocoxites lateral compressing (F–H). Genital supplement that have caused manhood regulators and you will gonocoxites compacted laterally, when you look at the dorsal (I) and you will ventral glance at (J).
Practical morphology of your own male genital capsule
A man genitalia of Yards. fasciculata has step three section: dos overlapping metasomal sterna (S-VIII and you will S-IX) and a genital supplement ( Figure dos). The fresh genital supplement enjoys a pair of almost triangular gonocoxites, where a set of a lot of time gonostyli as well as the collection of sharp penis regulators is articulated; dorsally there can be a slim gonobase and you will a median spatha ( Shape 2E,I). These types of sclerites is strongly stored with her because of the a series of walls that enable the totally free articulation. Every areas try firmly sclerotized, except for the fresh putatively delicate setae towards distal third off gonostyli; consequently they are organized ventrally in the men looks.
The female genitalia off Meters. fasciculata is positioned inside a beneficial chamber molded from the tergum and sternum of one’s sixth intestinal portion ( Contour step three). The final part of the women body’s membranous, poorly sclerotized, stored by pairs of lateral hemitergites (T-VIII and you may T-IX). Interestingly due to the fresh new membranous rear-end, we see a couple of brief setose gonostyli, a central light bulb (the fresh new gonopore town), and a pair of thin, lateral rami. Anywhere between these types of formations and the sternum S-VI below, following next the latest gonopore city, there are highest membranous pouches-muscle purse with just minimal sclerotization-onto that the men mating plug is actually attached in mating ( Shape 3A,J) and copulatory marks are observed ( Additional Shape S5 ). Membranous purse was depicted of the a light room beneath 
the gonopore inside the Shape 3. These membranous formations are shown inside the Figure step one for all variety, both in virgin and you may mated queens, regardless if their cousin dimensions and you may positioning can be seen in detail within the Additional Figure S5 .
